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ABSTRACT

Bacterial transformation is the process by which the bacteria’s function is altered by the integration of external DNA, or plasmids. This process is commonly used to control the genetic traits expressed by the organisms transformed, such as in the use of bacteria to produce human-viable insulin. Electromagnets are induced magnets created when an electrical current is passed through a ferromagnetic material. A magnetic field in which magnetic effects can be felt exists around this electromagnet. The purpose of this investigation was to determine whether there was a significant relationship between the presence of an applied electromagnetic field, and the transformation rate of bacteria. It was hypothesized that the presence of the electromagnetic field would increase the rate of transformation. This was tested by transforming the E. coli K12 using the pGlo plasmid. Three cultures of the transformed bacteria were incubated at 37ºC on nutrient agar with the antibiotic ampicillin between two solenoid electromagnets, and three other cultures were incubated without the electromagnets. Due to complications during the transformation process (the transformation process was most likely done improperly), no results have been found as none of the bacterial cultures grew any colonies. For future research, the transformation process will be revised.

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