The discovery that certain South American leaf cutter ant species maintain healthy and uninfected fungus farm through a symbiotic relationship with actinomycete bacteria has prompted a search for possible path toward developing a new antibiotic or antimicrobial compounds. The development of antibiotics using these compounds would enable a path toward slowing the advancement of antibiotic resistance. Through the investigations of the functions and effects that the bacteria have on the ant colonies, it becomes evident that the bacteria that have had the most significant impact on the inhibition of growth of surrounding bacteria species were the actinomycetes Streptomyces, whose relatives have previously been used to produce antibiotics, and Pseudonocardia. It was also found that the system that the ant species developed to protect their colony’s food supply suggest that they are able to chemically prompt the Pseudonocardia to enter their colony and live on their fungi compost piles, as well as join their colony. These studies have also shown evidence that the ants and symbiotic bacteria coevolved, which means that they have unique properties and would therefore be more effective when developing antibacterial substances. These conclusions allow for the development of a procedure to derive antimicrobial compounds from the Pseudonocardia and Streptomyces bacteria and observe the zone of inhibition when the compounds are placed inside petri dishes containing other species. What will be observed is a ring around the antibiotic disc where none of the other bacteria species (either S. aureus or E. coli) shows any colony growth or coverage.